Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine.

نویسندگان

  • Shinsuke Ito
  • Li Shen
  • Qing Dai
  • Susan C Wu
  • Leonard B Collins
  • James A Swenberg
  • Chuan He
  • Yi Zhang
چکیده

5-methylcytosine (5mC) in DNA plays an important role in gene expression, genomic imprinting, and suppression of transposable elements. 5mC can be converted to 5-hydroxymethylcytosine (5hmC) by the Tet (ten eleven translocation) proteins. Here, we show that, in addition to 5hmC, the Tet proteins can generate 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) from 5mC in an enzymatic activity-dependent manner. Furthermore, we reveal the presence of 5fC and 5caC in genomic DNA of mouse embryonic stem cells and mouse organs. The genomic content of 5hmC, 5fC, and 5caC can be increased or reduced through overexpression or depletion of Tet proteins. Thus, we identify two previously unknown cytosine derivatives in genomic DNA as the products of Tet proteins. Our study raises the possibility that DNA demethylation may occur through Tet-catalyzed oxidation followed by decarboxylation.

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عنوان ژورنال:
  • Science

دوره 333 6047  شماره 

صفحات  -

تاریخ انتشار 2011